Sarcocystis spp. detection in cattle using different diagnostic methods

ABSTRACT: Cattle are considered intermediate hosts of Sarcocystis, which can cause clinical signs and lower performance in the acute phase infection. Sarcocystis spp. usually not visible to naked eye during post mortem inspection. Moreover, fresh microscopic examination transmission electron microscopy techniques difficult apply large samples. Therefore, extensive studies on infection cattle using molecular serological methods required. Here, we investigated polymerase chain reaction myocardium samples compared results with presence antibodies against corresponding serum detected indirect fluorescent antibody test. Microscopic were observed 100% myocardial samples, DNA was present 86% (43/50) these Antibodies 96% (48/50) 80% (40/50) at 1:25 1:200 dilutions, respectively. The three associated (fresh examination, PCR serology) showed good sensitivity detection for (only), they may facilitate diagnosis live animals a scale as well monitoring herd status.